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In addition, one member of the genus Eurotium, which includes moderately xerophilic species, was identified. For example, Diamond Painting tradition-unbiased strategies allowed the detection of different genera, such as the genus Stenotrophomonas or the genus Microbacterium, which could not be detected using cultivation strategies, as beforehand observed within the research performed by Pepe et al. In abstract, essentially the most incessantly remoted bacterial and fungal strains belonged to Bacillus-related species and to the genus Penicillium, respectively.

DNA from pure fungal strains existing predominantly as mycelia, nevertheless it proved to be ineffective in spore lyses. On the contrary, non-spore-forming bacteria belonging to the phylum Proteobacteria couldn’t be isolated by cultivation strategies however had been shown to be dominant when total DNA was straight extracted from canvas material (pattern VG4). Three fragments of an early fifteenth-century Italian fresco, The Fall of Lucifer by Spinello Aretino, had been offered to the Nationwide Gallery, London by Sir Henry Layard in 1886.

They have been detached from the wall above the high altar of Compagnia di Sant’Angelo, or S. Michele Arcangelo, in Arezzo by the strappo approach and lined onto canvas. However, the mordant securing the tin-leaf decoration was found to contain oil and sandarac resin, and there was uncertainty in regards to the impact of extended immersion in PET (used to remove the CDD after therapy) on the mordant.